What is FRET in fluorescence microscopy? What can FRET be used for in molecular biology?



Question 1)

What is FRET in fluorescence microscopy? What can FRET be used for in molecular biology?

Question 2)

Atomic Force Microscopy is a useful technique for probing molecular interactions

  1. How does an AFM work? Draw a sketch.
  2. You want to look at distribution of a protein localized on the surface of a microbial cell. How would you do that with an AFM with a gold-coated tip?

Question 3) 

  1. What is hydrodynamic focusing in a microfluidic device, what does the design look like and what can it be used for?
  2. What is microcontact printing? Outline the process of fabricating a microcontact printing stamp in PDMS.

Question 4)

What problems are associated with attaching proteins to a surface, particularly in the nanoscale?

Question 5)

You are approached by an immunologist who needs help to design a device to study cell-cell interactions at single cell-pair level for experiments lasting a few hours. Use your skills learned in this course and your imagination to help the immunologist design a cell-pairing device.


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